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Pharmacia LKB Biotechnology Inc
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Aero-Laser GmbH
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Chemie GmbH
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EdgeWave GmbH
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TOPTICA Photonics
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Silixa Ltd
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Optik GmbH
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Verlag GmbH
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Trimble Navigation
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CVI Laser Optics
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Becton Dickinson
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CapitalBio Corporation
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Image Search Results
Journal: Journal of Neuroscience Methods
Article Title: Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope
doi: 10.1016/j.jneumeth.2013.10.021
Figure Lengend Snippet: A compact acousto-optic lens (AOL) microscope for two-photon functional imaging in 3D at 36 kHz bandwidth. (A) Main optical components of the AOL microscope: a femtosecond laser source, a customized prechirper to compensate for temporal dispersion, a beam expander, 4 custom-designed acousto-optic deflector (AOD) crystals in series, together with a conventional 2-photon microscope including photomultiplier tube (PMT) detection. Cartoon below showing focusing and steering of a laser beam to 3 points of interest (POI) within neural tissue. Inset AOL efficiency (fraction of laser power transmitted through the AOL) during operation of the laser at wavelengths used in neuroscience applications. (B) Illustration of remote focusing of laser beam with two AODs. Linearly varying sound waves created by ultrasonic transducers (grey sheets) transformed the planar optical wavefront into a curved wavefront, thereby focusing the beam in the Z axis. A counter-propagating acoustic wave in the second crystal transiently compensates for movements in the X axis, enabling the laser beam to be transiently focused in the XZ plane. (C) Cartoon illustrating random access measurements from 5 POIs. It takes 28 μs to revisit each point (24 μs for a sound wave to fill the AOD plus 4 μs dwell time), giving a total serial bandwidth of 36 kHz, which divided across 5 selected POIs is 7 kHz.
Article Snippet: The AOL was constructed of 4
Techniques: Microscopy, Functional Assay, Imaging, Transformation Assay